Thermo Fisher Scientific ZHX2 Polyclonal Antibody
ZHX2 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체. Western blot, IHC, ICC/IF, IP, ChIP-Seq 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. 인체 유래 단백질 검출에 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 533–826 of ZHX2 (Uniprot ID: Q9Y6X8) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.70 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152853 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji
- Predicted reactivity: Bovine (80%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1–3 are ubiquitously expressed proteins found in various tissues. They act as transcriptional repressors and localize to the nucleus.
ZHX proteins contain two Cys(2)-His(2)-type zinc-finger motifs and five homeodomains (HDs), enabling homodimer and heterodimer formation. However, dimerization is not required for repressor activity.
Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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