Thermo Fisher Scientific ATP6V1F Monoclonal Antibody (OTI1B8), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is essential for processes such as:

• Protein sorting
• Zymogen activation
• Receptor-mediated endocytosis
• Synaptic vesicle proton gradient generation

V-ATPase consists of:

• V1 domain (cytosolic): three A and three B subunits, two G subunits, and C, D, E, F, H subunits (contains ATP catalytic site)
• V0 domain (transmembrane): five different subunits (a, c, c′, c″, and d)

Additional isoforms of many V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants.
This encoded protein is the V1 domain F subunit protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.