Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test
• View 3 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant Violet 480
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) expressed by activated α/β-TCR bearing T cells, a subset of γ/δ-TCR T cells, and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein structurally similar to CD4. A soluble form has been detected in human serum, and mouse CD223 can be proteolytically cleaved into a 54 kDa extracellular fragment and a 16 kDa intracellular fragment. The 54 kDa fragment may remain membrane-bound or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4 and is thought to negatively regulate T-cell activation and homeostatic proliferation. It is also expressed by CD4+CD25+ regulatory T cells and may contribute to their regulatory function.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.

Fluorochrome Information:

• Excitation: 405 nm
• Emission: 436 nm
• Recommended filter: 450/50 bandpass
• Laser: Violet Laser

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

LAG-3 is a 70 kDa surface glycoprotein of the Ig superfamily, homologous to CD4. It binds MHC class II with higher affinity than CD4 and is involved in negative regulation of T-cell activation and homeostasis. It is expressed on activated T cells (including regulatory T cells) and NK cells, with CD8+ T cells typically showing higher expression levels. Coexpression of LAG-3 and CD49b identifies Type 1 regulatory T cells (Tr1) in human and mouse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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