Thermo Fisher Scientific TNP2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

During mammalian spermiogenesis, histones are transiently replaced by several low molecular weight proteins called transition proteins (TNPs). These proteins facilitate chromatin transformation from the nucleosome to the nucleoprotamine structure during spermatid differentiation.
Transition protein-2 (TNP2), also known as TP2, maps to human chromosome 16p13.13 and encodes a highly basic nuclear protein. TNP2 is a spermatid-specific product of the haploid genome that replaces histones and is later replaced by protamines in mature sperm.
Although TNP2 is not essential for sperm nuclear shaping, histone displacement, or fertility, it is required for proper processing of protamine 2 and completion of chromatin condensation. In the absence of TNP1, TNP2 may partially compensate, but this imbalance can result in abnormal chromatin condensation and reduced fertility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.