Thermo Fisher Scientific DNase I Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

Internucleosomal DNA fragmentation following activation of endonucleases is a hallmark of apoptosis. DNase I, a Ca²⁺/Mg²⁺-dependent endonuclease ubiquitously expressed in mammalian tissues, mediates internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, with at least six known alleles.
DNase II, an acidic deoxyribonuclease, functions downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5′-phosphodinucleotide and 5′-phosphooligonucleotide end-products, whereas DNase II cleaves DNA to 3′-phosphomononucleotide and 3′-phosphooligonucleotide end-products.
DNase II is typically found in the cytoplasm of epithelial cells but is concentrated in the nuclei of lens fibers. In contrast, DNase I is consistently localized in nuclei of epithelial and fiber cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.