Thermo Fisher Scientific CD16b Polyclonal Antibody
Thermo Fisher Scientific의 CD16b Polyclonal Antibody는 인간 및 랫트 시료에 반응하는 Rabbit IgG 항체로, Western blot과 ELISA에 적합합니다. CD16b 단백질(FCGR3B)을 인식하며, 세포막 단백질 연구 및 면역학 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–233 of human FCGR3B (NP_000561.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.88 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805650 |
Product Specific Information
Immunogen sequence:
MWQLLLPTAL LLLVSAGMRT EDLPKAVVFL EPQWYSVLEK DSVTLKCQGA YSPEDNSTQW FHNENLISSQ ASSYFIDAAT VNDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKDRKY FHHNSDFHIP KATLKDSGSY FCRGLVGSKN VSSETVNITI TQGLAVSTIS SFSPPGYQVS FCLVMVLLFA VDTGLYFSVK TNI
Positive Samples: THP-1, Rat thymus
Cellular Location: Cell membrane, GPI-anchor, Lipid-anchor
Target Information
CD16 (FCGR3A) is a 50–65 kDa cell surface molecule that exists in two forms: a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol-linked form expressed by granulocytes.
CD16 is a low-affinity receptor for IgG (FcR III) and plays a key role in antibody-dependent cellular cytotoxicity (ADCC) mediated by NK cells.
Human CD16 exists in two forms:
- FCGR3A: Associated with the FcεRI-γ subunit, responsible for NK cell cytotoxicity
- FCGR3B: GPI-linked monomeric receptor expressed on neutrophils, involved in activation and pro-adhesive phenotype induction
Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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