Thermo Fisher Scientific REA Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 사용 가능한 Rabbit Polyclonal 항체. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. Recombinant Prohibitin 2 단백질(aa 45–299)을 면역원으로 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 45–299 of Prohibitin 2 (Uniprot ID: Q99623) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.66 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152372 |
Product Specific Information
- Recommended positive controls: H1299, HeLa, mouse brain
- Predicted reactivity:
Mouse (100%), Rat (100%), Xenopus laevis (87%), Pig (98%), Chicken (95%), Rhesus Monkey (100%), Bovine (99%) - Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases.
This protein functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens.
It competes with NCOA1 for modulation of ER transcriptional activity and is likely involved in regulating mitochondrial respiration activity and aging.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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