Thermo Fisher Scientific HIV1 Vif Monoclonal Antibody (319)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

319

Immunogen

Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 Vif protein (clade B, HXB-3 isolate)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539413

Product Specific Information

This target has a predicted molecular weight of 23 kDa.

Target Information

The HIV-1 viral infectivity factor (Vif) is a crucial protein for the efficient replication of the virus, particularly in non-permissive cells such as lymphocytes and macrophages. Vif is a basic phosphoprotein with a molecular weight of approximately 23,000 Daltons. Its primary function is to counteract the host cellular antiviral protein APOBEC3G, which induces hypermutations in the viral DNA, thereby inhibiting viral replication. Vif achieves this by targeting APOBEC3G for ubiquitination and subsequent degradation, thus preventing it from incorporating into new virions. Structurally, Vif interacts with the cellular ubiquitin-proteasome machinery to facilitate the degradation of APOBEC3G. This interaction is essential for Vif`s role in viral replication and immune evasion. Additionally, Vif has been shown to suppress the production of type I interferon by targeting the immune signaling molecule STING, further aiding in immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.