Thermo Fisher Scientific GRP94 Polyclonal Antibody
GRP94 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. Horse, Human, Mouse, Non-human primate 반응성. Protein G 정제 및 PBS buffer(0.2% gelatin, 0.05% sodium azide) 포함. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Horse, Human, Mouse, Non-human primate |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an internal portion of the human GRP94 protein (between amino acids 550–650) [UniProt P14625] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.2% gelatin |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2540703 |
Target Information
GRP94 is an 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone involved in processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulate CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate and acquired immunity, dendritic cell maturation and chemotaxis, antibody production, and cross-priming. GRP94 is also a potential marker in breast cancer and functions as a peptide acceptor in the endoplasmic reticulum, assisting peptide loading of MHC class I molecules. Expression of GRP94 suppresses A23187-induced apoptosis and stabilizes calcium homeostasis. It is expressed in melanoma, liver metastases of colon carcinoma, and human gastric carcinoma BGC-823 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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