Thermo Fisher Scientific Calcineurin B/PPP3R1 Monoclonal Antibody (CALNB, 2342)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase essential in the T cell activation pathway. It consists of two subunits:

• Calcineurin A (CnA): 60 kDa catalytic subunit, homologous to protein phosphatases 1 and 2A.
• Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium binding sites.

Calcineurin-mediated dephosphorylation of NF-AT is crucial for its activation and nuclear translocation. Immunosuppressive drugs such as cyclosporin A and FK506 inhibit this process by binding to immunophilins (cyclophilin and FKBP), forming complexes that block substrate access to the catalytic domain of CnA without inhibiting its phosphatase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.