Thermo Fisher Scientific DDX3 Polyclonal Antibody
DDX3 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 적합합니다. Human에 반응하며, 예측 반응종으로 Mouse, Pig, Chicken 등 포함. 항원 친화 크로마토그래피로 정제되었으며, PBS(glycerol 포함) 용액 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 254–521 of Human DDX3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.87 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2546646 |
Product Specific Information
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, U87MG, U87-MG Cytoplasm, Human ESC
- Predicted reactivity: Mouse (98%), Xenopus laevis (97%), Pig (100%), Chicken (98%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. It exhibits ATP-dependent RNA helicase activity and RNA-independent ATPase activity. Unlike most DEAD-box helicases, its ATPase activity is stimulated by both RNA and DNA.
This protein functions in both nucleus and cytoplasm—participating in transcriptional regulation, mRNP assembly, pre-mRNA splicing, mRNA export, translation, cellular signaling, and viral replication. Misregulation has been implicated in tumorigenesis. The gene has a paralog on the Y chromosome, and pseudogenes are found on chromosome 4 and the X chromosome. Alternative splicing produces multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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