Thermo Fisher Scientific IL-6 Monoclonal Antibody (7IL6), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Dog, Human, Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7IL6

Immunogen

Recombinant human IL-6.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223577

Product Specific Information

M621B targets IL-6 in ELISA and WB applications and shows reactivity with Human samples.

The M621B immunogen is recombinant human IL-6.

M621B detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

The M621B IL6 antibody (biotinylated conjugate of clone 7IL6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M620 (clone 5IL6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M621B (clone 7IL6) and antibody M620 (clone 5IL6) have successfully been used in combination with recombinant IL6 protein SIL6 in ELISA applications.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.