Thermo Fisher Scientific Complement C5b Monoclonal Antibody (aE11)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

1:20,000

-

Product Specifications

Species Reactivity

Horse, Human, Non-human primate, Pig

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

aE11

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.04 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2063730

Product Specific Information

DIA 011-01-02 binds both membrane-bound MAC and fluid-phase SC5b-9 complexes from human, porcine, baboon and equine samples.

DIA 011-01-02 has been successfully used in ELISA and IHC applications. This antibody is not recommended for Western blotting as the epitope is destroyed during the process.

Target Information

C5b-9 is also known as the TCC. TCC consists of C5b, C6, C7, C8 and C9. The complex forms transmembrane channels which displace lipid molecules and other constituents, thus disrupting the phospholipid bilayer of target cells leading to cell lysis by osmotic leakage. The complex contains neoantigens that are absent from the individual native components and the monoclonal antibody DIA 011-01 is directed against a neoepitope exposed in C9 when incorporated into TCC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.