Thermo Fisher Scientific Complement C5b Monoclonal Antibody (aE11)
C5b-9 복합체를 인식하는 단클론 항체로, 인간, 말, 돼지, 비인간 영장류 시료에서 반응합니다. ELISA 및 IHC에 적합하며, Western blot에는 비추천됩니다. 액상 형태로 1.04 mg/mL 농도이며, PBS 완충액에 보존됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| ELISA | 1:20,000 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Horse, Human, Non-human primate, Pig |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | aE11 |
| Immunogen | Purified C5b-9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.04 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2063730 |
Product Specific Information
DIA 011-01-02 binds both membrane-bound MAC and fluid-phase SC5b-9 complexes from human, porcine, baboon and equine samples.
It has been successfully used in ELISA and IHC applications.
This antibody is not recommended for Western blotting as the epitope is destroyed during the process.
Target Information
C5b-9, also known as the terminal complement complex (TCC), consists of C5b, C6, C7, C8, and C9.
This complex forms transmembrane channels that disrupt the phospholipid bilayer of target cells, leading to cell lysis by osmotic leakage.
The complex contains neoantigens absent from the individual native components.
The monoclonal antibody DIA 011-01 is directed against a neoepitope exposed in C9 when incorporated into TCC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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