Thermo Fisher Scientific Human IgE Isotype Control (HE1)

Applications

Tested Dilution

Immunohistochemistry (IHC)

Assay-Dependent

Control (Ctrl)

Assay-dependent

Product Specifications

Host/Isotype

Human / IgE, kappa

Class

Monoclonal

Type

Isotype Control

Clone

HE1

Immunogen

monoclonal hybridoma from a healthy donor

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein L

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10979124

Product Specific Information

DIA HE1-01A contains non-immune purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B, but must be handled as potentially infectious as all human material.

The purity of this human IgE is >98%, tested by SDS-PAGE. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.

This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.