
Thermo Fisher Scientific E2F5 Polyclonal Antibody
Human E2F5 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot, IP, ChIP assay에 적합. 보존제가 없는 액상 형태로 제공되며, 장기 보관 시 -20°C 권장. 세포주기 조절 및 종양 억제 단백질 연구에 활용 가능.
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Thermo Fisher Scientific E2F5 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunoprecipitation (IP) | 1–2 µL/mg of lysate | – |
| ChIP assay (ChIP) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human E2F5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788401 |
Product Specific Information
- Preservative-free formulation.
- Recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
- Specificity: Human E2F5.
Target Information
E2F5 is a member of the E2F family of transcription factors, which play a crucial role in cell cycle control and tumor suppressor protein action. The E2F proteins contain conserved domains, including:
- DNA binding domain
- Dimerization domain for DP protein interaction
- Transactivation domain rich in acidic amino acids
- Tumor suppressor protein association domain
E2F5 is differentially phosphorylated and expressed in various human tissues. It shares high identity with E2F4 and interacts with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing leads to multiple isoforms.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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