Thermo Fisher Scientific Histone H1.2 Recombinant Rabbit Monoclonal Antibody (JU43-48)
Histone H1.2 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ChIP 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 제조되었습니다.
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Thermo Fisher Scientific Histone H1.2 Recombinant Rabbit Monoclonal Antibody (JU43-48)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ChIP assay (ChIP) | 0.5–2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU43-48 |
| Immunogen | Synthetic peptide within Human Histone H1.2 (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810088 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened for optimal production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
Histones are basic nuclear proteins that form the nucleosome structure of chromosomal fibers in eukaryotes. Each nucleosome consists of an octamer of core histones (H2A, H2B, H3, H4) wrapped by ~146 bp of DNA.
Histone H1 interacts with linker DNA between nucleosomes, contributing to chromatin compaction. The Histone H1.2 gene is intronless, lacks polyA tails, and resides in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(MA5-32812_Histone_H1.2_P16403-1_Rabbit_PDP.jpeg, MA5-32812_Histone_H1.2_P16403-1_Rabbit.svg)
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