Thermo Fisher Scientific CCL2 (MCP-1) Monoclonal Antibody (2H5), eBioscience
CCL2(MCP-1) 단백질을 인식하는 2H5 클론 단일클론 항체로, 인간·마우스·랫트 반응성이 있음. ELISA, Flow cytometry, Neutralization 등 다양한 분석에 사용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 안정적이며, 액상 형태로 4°C 보관.
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Thermo Fisher Scientific CCL2 (MCP-1) Monoclonal Antibody (2H5), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | Assay-Dependent | View 1 publication |
| ELISA | 0.5–2 µg/mL | View 1 publication |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468430 |
Additional Formats:
Product Specific Information
Description:
The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.
Applications Reported:
The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization.
For functional assays, use Functional Grade purified 2H5 antibody (Product # 16-7096).
Applications Tested:
Mouse MCP-1 ELISA:
Biotinylated 2H5 antibody serves as the detection antibody in a sandwich ELISA with affinity purified 4E2/MCP (Product # 14-7091-81) as capture and recombinant mouse MCP-1 as standard.
Recommended concentration range: 0.5–2.0 µg/mL.
Include a standard curve from 2000 pg/mL to 15 pg/mL.Human MCP-1 ELISA:
Biotinylated 2H5 antibody serves as the detection antibody in a sandwich ELISA with affinity purified 5D3-F7 (Product # 14-7099-81) as capture and recombinant human MCP-1 as standard.
Recommended concentration range: 0.5–2.0 µg/mL.
Include a standard curve from 2000 pg/mL to 15 pg/mL.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
This gene encodes a cytokine located on chromosome 17q, part of a cluster of immunoregulatory and inflammatory cytokine genes.
The protein is structurally related to the CXC subfamily, characterized by two cysteines separated by one amino acid.
CCL2 exhibits chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils.
It is implicated in diseases with monocytic infiltration such as psoriasis, rheumatoid arthritis, and atherosclerosis.
CCL2 binds to chemokine receptors CCR2 and CCR4.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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