Thermo Fisher Scientific Histone H4 Recombinant Rabbit Monoclonal Antibody (ARC2340)
Histone H4 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA에 적합합니다. 인간, 생쥐, 랫트 시료에 반응하며, HEK293 세포에서 발현되었습니다. 고순도 친화 크로마토그래피 정제 및 안정한 PBS/glycerol buffer로 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC2340 |
| Immunogen | Synthetic peptide corresponding to amino acids 24–103 of human Histone H4 (P62805) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897935 |
Product Specific Information
- Positive Samples: HeLa, NIH/3T3, C6
- Immunogen Sequence:
RDNIQGITKP AIRRLARRGG VKRISGLIYE ETRGVLKVFL ENVIRDAVTY TEHAKRKTVT AMDVVYALKR QGRTLYGFGG
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction. The Histone H4 gene is intronless, lacks a polyA tail, and is located in the large histone gene cluster on chromosome 6.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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