Thermo Fisher Scientific Alginate Monoclonal Antibody (3G4-1F5), FITC
FITC로 표지된 알지네이트 단클론 항체로, Western blot 및 IHC에 사용 가능. 마우스 IgG1, kappa 아이소타입. 단백질 G로 정제된 액상 형태로 제공되며, 4°C 암소 보관. 세포 고정화 및 단백질 전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G4-1F5 |
| Immunogen | Sodium Alginate conjugated to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931770 |
Additional Formats
- Unconjugated (Cat. MA5-27564)
- APC (Cat. MA5-45315)
- PE (Cat. MA5-45318)
- PerCP (Cat. MA5-45317)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45316 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.
Target Information
Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca²⁺.
Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds and in various cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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