Thermo Fisher Scientific PABP Monoclonal Antibody (10E10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Chicken, Human, Rabbit, Xenopus

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

10E10

Immunogen

Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2156879

Product Specific Information

MA1-34079 detects PABP from human, chicken, rabbit and Xenopus laevis samples. Does not react wtih Drosophilia melanogaster or mouse samples.

Target Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.