Thermo Fisher Scientific Phospho-S6 (Ser240, Ser244) Polyclonal Antibody
Phospho-S6 (Ser240, Ser244) 항체로, 인산화된 RPS6 단백질 검출에 적합. Western blot, IHC, ELISA, IP 등 다양한 응용 가능. 사람, 생쥐, 랫트 반응성. Rabbit polyclonal IgG로 제작되었으며, 고순도 친화 크로마토그래피 정제. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_0010012) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.29 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901829 |
Product Specific Information
- Positive Samples: HeLa, NIH/3T3
- Immunogen sequence: STSKS
Target Information
Ribosomes are organelles responsible for protein synthesis, composed of a small 40S subunit and a large 60S subunit. These subunits contain 4 RNA species and about 80 distinct proteins. The encoded protein, a component of the 40S subunit, belongs to the S6E family of ribosomal proteins. It serves as a major substrate for ribosomal protein kinases, with phosphorylation on multiple C-terminal serine residues induced by stimuli such as growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs during growth arrest, suggesting a role in regulating cell growth and proliferation through selective mRNA translation. Multiple processed pseudogenes of this gene exist throughout the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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