Thermo Fisher Scientific HNMT Polyclonal Antibody
Thermo Fisher Scientific의 HNMT Polyclonal Antibody는 인간 및 마우스 시료에 반응하며 Western blot, ICC/IF, ELISA, IP에 사용 가능합니다. 항원은 인간 HNMT의 1-292 아미노산 서열로부터 제조되었으며, 액상 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–292 of human HNMT (NP_008826.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.41 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855236 |
Product Specific Information
Immunogen sequence:
MASSMRSLFS DHGKYVESFR RFLNHSTEHQ CMQEFMDKKL PGIIGRIGDT KSEIKILSIG GGAGEIDLQI LSKVQAQYPG VCINNEVVEP SAEQIAKYKE LVAKTSNLEN VKFAWHKETS SEYQSRMLEK KELQKWDFIH MIQMLYYVKD IPATLKFFHS LLGTNAKMLI IVVSGSSGWD KLWKKYGSRF PQDDLCQYIT SDDLTQMLDN LGLKYECYDL LSTMDISDCF IDGNENGDLL WDFLTETCNF NATAPPDLRA ELGKDLQEPE FSAKKEGKVL FNNTLSFIVI EA
Target Information
Histamine in mammals is metabolized via two major pathways:
- N(tau)-methylation via histamine N-methyltransferase (HNMT)
- Oxidative deamination via diamine oxidase
HNMT is a cytosolic enzyme that uses S-adenosyl-L-methionine as a methyl donor. In the brain, histamine neurotransmitter activity is regulated by N(tau)-methylation since diamine oxidase is absent in the central nervous system. A common genetic polymorphism can affect HNMT activity in red blood cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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