Thermo Fisher Scientific Phospho-RRAS (Tyr66) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of R-Ras only when phosphorylated at Tyr66.

Target Information

The Ras superfamily of small GTPases includes the Ras, Rho, Arf, Rab, and Ran families. Ras-regulated signaling pathways control actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. These GTPases function as molecular switches that regulate eukaryotic cell processes by cycling between GDP-bound inactive and GTP-bound active forms. GTP binding induces conformational changes in the effector-binding domain. Ras proteins are monomeric GTPases (20–30 kDa) attached to the cell membrane by prenylation. Mutations in Ras family proto-oncogenes (H-Ras, N-Ras, K-Ras) are among the most frequent in human cancers, occurring in 20–30% of all tumors. K-Ras mutations are common in non-small-cell lung, colorectal, and pancreatic carcinomas; H-Ras in bladder, kidney, and thyroid carcinomas; N-Ras in melanoma, hepatocellular carcinoma, and hematologic malignancies.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.