Thermo Fisher Scientific N4BP1 Polyclonal Antibody
Human N4BP1 단백질에 특이적인 Rabbit Polyclonal 항체로, IHC(P)에서 1:500~1:1,000 희석 배수로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS와 글리세롤 버퍼에 보관. Nedd4 관련 단백질 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human N4BP1. Recombinant protein control fragment (Product #RP-98672) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644411 |
Product Specific Information
Immunogen sequence:
QEEARNKAGT PVSELTKQMD TVLSSSPDVL FDPINGLTPD EEALSNERIC QKRRFSDSEE RHTKKQFSLE NVQEGEILHD AKTLAGNVIA DLSDSSADSE NLSPDI
Highest antigen sequence identity to the following orthologs:
- Mouse: 72%
- Rat: 73%
Target Information
Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role in embryonic processes.
N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. It also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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