Thermo Fisher Scientific H2AX Polyclonal Antibody

Applications

Immunohistochemistry (IHC)

• Tested Dilution: 1:100–1:500

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Guinea pig, Pig, Monkey, Gorilla, Little brown bat, Thirteen-lined ground squirrel, and Chinese hamster.

Target Information

Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle.
In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the C-terminus of H2AX, resulting in γ-H2AX formation.
This phosphorylation can be detected by Western blot or immunofluorescence to assess DNA damage frequency.
Phosphorylation is mediated by phosphatidylinositol 3-kinases, potentially involving ATM, DNA-PK, and ATR.
H2A.X contains 143 amino acids and is synthesized during both G1 and S phases.
Histone H2A.X maps to the 11q23.2–q23.3 region of the human chromosome and plays a key role in DNA structure and repair mechanisms.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.