Thermo Fisher Scientific LEF1/TCF1 alpha (Transcription Factor) Recombinant Rabbit Monoclonal Antibody (LEF1, 341R)
인간 LEF1 단백질을 표적으로 하는 재조합 토끼 모노클로날 항체로, IHC(P)에서 1–2 µg/mL 농도로 사용 가능. HEK293 세포에서 발현되었으며, Protein A/G로 정제됨. Wnt 신호전달 및 모발 세포 분화 연구에 활용 가능.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | LEF1, 341R |
| Immunogen | Recombinant fragment (around aa100–200) of human LEF1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The encoded protein binds to a functionally important site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors and are linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(해당 항체의 면역원 구조 이미지: LEF1, Q9UJU2-1, Rabbit)
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