Thermo Fisher Scientific RHD Recombinant Human Monoclonal Antibody (D7C2)
인간 RHD 항원에 특이적인 재조합 인간 단일클론 항체(D7C2)로, Flow Cytometry 및 Cytotoxicity Assay에 사용 가능. HEK293 세포에서 발현된 IgM 람다형 항체이며, 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: Assay-dependent
Cytotoxicity Assay (CT)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Human / IgM, lambda |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | D7C2 |
| Immunogen | A Rh- volunteer blood donor (r/r) was injected with 5 ml of Rh+ erythrocytes (R1/r). Peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
Target Information
The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans. Two highly homologous genes, RHD and RHCE (collectively referred to as RH30 or RHCED), encode the antigens of the Rh blood group system. These genes map to human chromosome position 1p34.1–1p36.
The RHD gene, often deleted in a large segment of the population, encodes the potent D antigen. Rh incompatibility between maternal and fetal blood types can cause hemolytic disease of the newborn (HDN), which may result in fetal death.
The RHCE gene exists in four allelic forms, determining the expression of antigens in Ce, ce, cE, or CE combinations. The RHCED antigens are integral membrane proteins with 12 transmembrane helices that maintain erythrocyte membrane integrity.
Presentation of Rh antigenic activity requires the formation of a complex between RHCED antigens and RHAG (RH50).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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