Thermo Fisher Scientific RHD Recombinant Human Monoclonal Antibody (D7C2)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

-

Cytotoxicity Assay (CT)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Human / IgM, lambda

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

D7C2

Immunogen

A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RHD,uniProtId:Q02161-1,ncbiNodeId:9606,antigenRange:1-417,antigenLength:417,antigenImageFileName:MA5-51821_RHD_Q02161-1_Human.svg,antigenImageFileNamePDP:MA5-51821_RHD_Q02161-1_Human_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Target Information

The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans. Two highly homologous genes RHD and RHCE (collectively referred to as RH30 or RHCED) encode the antigens of the Rh blood group system (1-4). These tightly linked genes map to human chromosomal position 1p34.1-1p36. The RHD gene, which is commonly deleted from a large segment of the population, encodes the most potent blood group immunogen, the D antigen (2,5). Rh incompatibility between maternal and fetal blood types results in hemolytic disease of the newborn (HDN), which often results in fetal death (1,2,6). The RHCE gene exists in four allelic forms, and each allele determines the expression of two antigens in Ce, ce, cE, or CE combinations (2,4,5). The RHCED antigens exist as integral membrane proteins with contain 12-transmembrane helices, and maintain erythrocyte membrane integrity. The presentation of the Rh antigenic activity requires the formation of a complex between the RHCED antigens and RHAG (RH50).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.