Thermo Fisher Scientific TLE1 Monoclonal Antibody (TLE1, 2051)
TLE1 단백질을 표적하는 Mouse Monoclonal 항체로, Western Blot과 IHC(Paraffin)에 적합. Recombinant human TLE1 fragment(aa 175-338)를 면역원으로 사용. 200 µg/mL 농도, Protein A/G 정제, PBS buffer에 0.05% BSA 및 sodium azide 포함. 연구용으로만 사용 가능.
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- 7088-MSM1-P1
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TLE1, 2051 |
| Immunogen | Recombinant human TLE1 fragment (aa 175–338) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Transcriptional corepressor that binds to a number of transcription factors.
Inhibits NF-kappa-B-regulated gene expression and transcriptional activation mediated by FOXA2, CTNNB1, and TCF family members in Wnt signaling.
The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
Unusual function as coactivator for ESRRG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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