Thermo Fisher Scientific alpha Internexin Monoclonal Antibody (2E3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:5,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:5,000

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E3

Immunogen

Purified recombinant rat a-internexin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_568590

Product Specific Information

The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so this antibody is ideal for studies of formalin-fixed paraffin-embedded histological sections.

Target Information

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.