Thermo Fisher Scientific SP1 Polyclonal Antibody
SP1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human에 반응하며 WB, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 완충액에 보관. 세포 성장, 분화, 면역 반응 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | 8 publications |
| Immunohistochemistry (IHC) | – | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | 1 publication |
| ChIP assay (ChIP) | Assay-dependent | 3 publications |
| Gel Shift (GS) | – | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 18–303 of Human SP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.22 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2546641 |
Product Specific Information
- Recommended positive controls: 293T, K562, THP-1, HL-60, Raji
- Predicted reactivity: Mouse (96%), Rat (99%), Rhesus Monkey (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in processes such as cell growth, apoptosis, differentiation, and immune responses. SP1 activity is highly regulated by post-translational modifications including phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may modulate cellular responses to DNA damage. SP1 is implicated in chromatin remodeling and plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter, being essential for FE65 gene regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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