Thermo Fisher Scientific MTOR Monoclonal Antibody (OTI3E5), TrueMAB
mTOR 단백질을 인식하는 Mouse 모노클로날 항체로 Western blot 및 IHC(P) 실험에 적합합니다. Human 시료에 반응하며, 정제된 Lyophilized 형태로 제공됩니다. 세포 성장 및 암 연구용으로 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1766–2144 of human MTOR produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation.
Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with various forms of cancer.
FRAP1 is the target of rapamycin and its analogues, which are used as immunosuppressants and cancer therapeutics.
Mutations in this gene are linked to Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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