Thermo Fisher Scientific Phospho-SLP76 (Ser376) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of SLP76 only when phosphorylated at Ser376.

Target Information

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat.
The SLP-76 locus has been localized to human chromosome 5q33 and partially characterized in mice.
Human and murine cDNAs encode 533 amino acid proteins that are 72% identical and composed of three modular domains:

• NH2-terminus: Acidic region with PEST domain and tyrosine residues phosphorylated after TCR ligation
• Central domain: Proline-rich region
• COOH-terminal domain: SH2 domain

SLP-76 interacts with multiple proteins both constitutively and inducibly following receptor ligation, functioning as an adaptor or scaffold protein.
Studies using SLP-76 deficient T cell lines or mice demonstrate its essential role in T cell development, activation, mast cell, and platelet function.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.