Thermo Fisher Scientific Phospho-Cdc20 (Ser51) Polyclonal Antibody
CDC20 단백질의 Ser51 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. 인간, 생쥐, 랫드 반응성. Western blot에 적합하며, 1 mg/mL 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDC20 (Accession Q12834), corresponding to amino acid residues around phosphorylated Ser51 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816671 |
Product Specific Information
Antibody detects endogenous levels of CDC20 only when phosphorylated at Ser51.
Target Information
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. It is regulated by MAD2L1: in metaphase the MAD2L1–CDC20–APC/C ternary complex is inactive, and in anaphase the CDC20–APC/C binary complex is active in degrading substrates. The CDC20–APC/C complex positively regulates the formation of synaptic vesicle clustering at the active zone to the presynaptic membrane in postmitotic neurons. CDC20–APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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