Thermo Fisher Scientific NEK3 Polyclonal Antibody
Human NEK3 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 IHC(Paraffin) 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 0.67 mg/mL 농도의 액상 형태로 제공됩니다. HepG2 세포를 양성 대조군으로 권장합니다.
- 카탈로그번호
- PA528569
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–255 of Human NEK3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.67 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris Glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546045 |
Product Specific Information
- Recommended positive controls: HepG2
- Predicted reactivity: Mouse (89%), Bovine (91%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3, and Nek4 (also designated STK2 or NRK2).
High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA. Like NIMA, Nek2 expression peaks at the G2 to M phase transition.
Nek3 is a predominantly cytoplasmic enzyme detectable in all organs studied. Levels of Nek3 seem to remain unchanged throughout the cell cycle but appear elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential expression patterns of Nek1, Nek2, and Nek4 indicate possible overlapping but non-identical functions.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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