Thermo Fisher Scientific Carbonic Anhydrase IX Polyclonal Antibody
Carbonic Anhydrase IX 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 등 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공되며, -20°C에서 보관.
- 카탈로그번호
- PA116592
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200–1:500 | – |
| Immunocytochemistry (ICC/IF) | 2–5 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | 1:1,000 | – |
| ELISA | Assay-Dependent | – |
| ChIP assay (ChIP) | 1:10–1:500 | – |
| in situ PLA (PLA) | Assay-Dependent | – |
| Gel Shift (GS) | Assay-Dependent | – |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Plant, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from a C-terminal sequence of the human CA IX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568476 |
Product Specific Information
- Suggested positive control: Antigen standard for CA9 (transient overexpression lysate), Renal carcinoma.
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in mammalian tissues and plays a key role in pH regulation by controlling hydrogen ion flux.
CA IX is highly inducible under hypoxic conditions via HIF-1 alpha and is located within the membrane. It serves as a reliable histochemical marker of hypoxia, as inhibition of CA IX increases cell death under hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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