Thermo Fisher Scientific Carbonic Anhydrase IX Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

2-5 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1:1,000

-

ELISA (ELISA)

Assay-Dependent

-

ChIP assay (ChIP)

1:10-1:500

-

in situ PLA (PLA)

Assay-Dependent

-

Gel Shift (GS)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Plant, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from a C-terminal sequence of the human CA IX. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Carbonic Anhydrase IX,uniProtId:Q16790-1,ncbiNodeId:9606,antigenRange:459,antigenLength:459,antigenImageFileName:PA1-16592_Carbonic_Anhydrase_IX_Q16790-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16592_Carbonic_Anhydrase_IX_Q16790-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568476

Product Specific Information

Suggested positive control: antigen standard for CA9 (transient overexpression lysate), Renal carcinoma.

Target Information

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.