Thermo Fisher Scientific Phospho-IkappaB epsilon (Ser161) Polyclonal Antibody
Phospho-IkappaB epsilon (Ser161) 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 인산화된 Ser161 위치의 IKB epsilon만을 검출합니다. 고순도 정제 및 안정적인 PBS/glycerol 버퍼로 보관됩니다.
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- PA5104688
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NFKBIE (Accession O00221), corresponding to amino acid residues around phosphorylated Ser161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816161 |
Product Specific Information
Antibody detects endogenous levels of IKB epsilon only when phosphorylated at Serine 161.
Target Information
NFKB1 or NFKB2 binds to REL, RELA, or RELB to form the NFKB complex.
The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which trap NF-kappa-B in the cytoplasm.
Phosphorylation of serine residues on I-kappa-B proteins by kinases (IKBKA or IKBKB) triggers their degradation via the ubiquitination pathway, leading to activation of the NF-kappa-B complex.
Activated NFKB translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5′-GGGRNNYYCC-3′ or 5′-HGGARNYYCC-3′.
For some genes, activation requires NFKB interaction with other transcription factors, including STAT, AP1, and NFAT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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