Thermo Fisher Scientific AES Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• This antibody is expected to recognize all reported isoforms (NP_945320.1; NP_001121.2; NP_945321.1).
• Tested in Peptide ELISA: antibody detection limit dilution 1:64,000.

Target Information

Adhesion to the extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/transducin-like enhancer of split (TLE) family of transcriptional regulators, which act as transcriptional co-repressors involved in neurogenesis, segmentation, and sex determination.
AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein released into the cytoplasm upon onset of apoptosis. This complex is thought to suppress survival-promoting gene transcription controlled by the TLE family. Interestingly, apoptosis of cells transfected with AES and Bit1 can be inhibited when cells attach to fibronectin via the α5β1 integrin, suggesting that the Bit1–AES pathway contributing to anoikis is regulated by integrins, particularly α5β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.