Thermo Fisher Scientific CD33 Monoclonal Antibody (OTI2G8), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit protein tyrosine phosphatases SHP-1 and SHP-2, mediating inhibitory signaling and receptor internalization.
CD33 is expressed on myelomonocytic lineage cells, binds sialic acid residues in N- and O-glycans, and serves as a therapeutic target for acute myeloid leukemia. It is present on granulocyte and macrophage precursors in bone marrow but absent from pluripotent stem cells, and is also expressed on peripheral monocytes.
CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Disorders associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.