Thermo Fisher Scientific IRS1 Polyclonal Antibody
Thermo Fisher Scientific의 IRS1 Polyclonal Antibody는 인간 및 마우스 IRS-1 단백질 검출용 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- PA11059
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 2 µg/mL
- Publications: View 2 publications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:250
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues D(631) Y M P M S P K S V S A P Q Q I(646) of human IRS-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325017 |
Product Specific Information
PA1-1059 detects IRS-1 in mouse and human cells.
It has been successfully used in Western blot procedures, detecting a ~170 kDa protein representing IRS-1 from 3T3 L1 cell extract.
The PA1-1059 immunogen is a synthetic peptide corresponding to residues D(631) Y M P M S P K S V S A P Q Q I(646) of human IRS-1, with 100% conservation in rats and mice.
This peptide (Cat. # PEP-193) is available for use in neutralization and control experiments.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4.
Phosphorylation occurs on serine, threonine, and tyrosine residues across various tissues.
Casein kinase II mediates a portion of insulin-stimulated serine/threonine phosphorylation of IRS-1 in vivo, with Thr 502 and Ser 99 identified as major phosphorylation sites.
IRS-1 contains three putative 14-3-3 binding sites (Ser 270, Ser 374, Ser 641), where the motif around Ser 270 is located in the phosphotyrosine binding domain responsible for interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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