Thermo Fisher Scientific IRS1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 2 µg/mL
• Publications: View 2 publications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:250

Product Specifications

Product Specific Information

PA1-1059 detects IRS-1 in mouse and human cells.
It has been successfully used in Western blot procedures, detecting a ~170 kDa protein representing IRS-1 from 3T3 L1 cell extract.
The PA1-1059 immunogen is a synthetic peptide corresponding to residues D(631) Y M P M S P K S V S A P Q Q I(646) of human IRS-1, with 100% conservation in rats and mice.
This peptide (Cat. # PEP-193) is available for use in neutralization and control experiments.

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4.
Phosphorylation occurs on serine, threonine, and tyrosine residues across various tissues.
Casein kinase II mediates a portion of insulin-stimulated serine/threonine phosphorylation of IRS-1 in vivo, with Thr 502 and Ser 99 identified as major phosphorylation sites.
IRS-1 contains three putative 14-3-3 binding sites (Ser 270, Ser 374, Ser 641), where the motif around Ser 270 is located in the phosphotyrosine binding domain responsible for interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.