Thermo Fisher Scientific APS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunocytochemistry (ICC/IF)

1:250

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues E(605) A T L G R A R A V E N Q Y S(619) from mouse APS. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:APS,uniProtId:Q9JID9-1,ncbiNodeId:10090,antigenRange:605-619,antigenLength:621,antigenImageFileName:PA1-1062_APS_Q9JID9-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1062_APS_Q9JID9-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2188849

Product Specific Information

PA1-1062 detects APS from mouse samples.

PA1-1062 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~ 84 kDa protein corresponding to APS from recombinant mouse protein and 3T3L1 extracts.

The PA1-1062 immunogen is a synthetic peptide corresponding to residues E(605)A T L G R A R A V E N Q Y S(619) from mouse APS. This peptide (Cat. # PEP-246) can be used in neutralization and control experiments.

Target Information

APS (adapter protein with Pleckstrin homology and Src homology 2 domains) a member of the Lnk family, is an adaptor protein that is involved in B-cell signaling, insulin signaling, cytoskeletal reorganization. It is tyrosine phosphorylated by JAK2, KIT and other kinases during B-cell receptor stimulation of many different cytokines, chemokines and leukokines. APS has been shown to inhibit the JAK-STAT pathway in collaboration with c-Cbl. It is located in the cytoplasm in pre-PDGF (platelet derived growth factor) stimulated cells and localizes to the plasma membrane and peripheral regions upon PDGF stimulation. This protein is expressed in B-cells and mast cells when detected in haematopoietic cell lines yet is also expressed in many different tissues including spleen, prostrate, testis, uterus, small intestine and skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.