Thermo Fisher Scientific ZBED1 Monoclonal Antibody (OTI1D5), TrueMAB
인간 ZBED1 단백질을 인식하는 마우스 단클론 항체로, Western blot에 적합합니다. HEK293T 세포에서 생산된 재조합 단백질을 면역원으로 사용하였으며, carrier-free 형태로 제공됩니다. DNA 복제 및 세포 증식 관련 연구용으로 최적화된 TrueMAB 제품입니다.
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Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D5 |
| Immunogen | Full-length human recombinant protein of human ZBED1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ZBED1 (also known as DREF) is the human homolog of the Drosophila DREF protein, a transcriptional regulator essential for the expression of genes involved in DNA replication and cell proliferation. RNAi targeting ZBED1 inhibits S-phase entry and reduces histone H1 mRNA levels in HeLa cells, indicating its role in cell proliferation.
ZBED1 binds promoter regions of at least 22 ribosomal protein genes, and its knockdown decreases RP gene expression. Immunoprecipitation studies show ZBED1 self-associates via its carboxyl-terminal hATC domain, which is required for nuclear localization and DNA binding.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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