Invitrogen™

pcDNA™3.2-DEST Mammalian Expression Vector

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers자세히 알아보기

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:

•Cytomegalovirus (CMV) promoter for high-level expression
•_att_R sites for Gateway™ cloning, enabling recombination with _att_L-flanked fragments
•C-terminal V5 tag for easy detection
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have _att_R sites for recombination with any _att_L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

사양

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

프로모터CMV

제품 유형Mammalian Expression Vector

선택 제제(진핵)Geneticin™ (G-418)

단백질 태그V5 Epitope Tag

클로닝 방법Gateway

수량6 μg

벡터pDEST, pcDNA

제품라인Gateway™, pcDNA™

용도(애플리케이션)Constitutive Expression

Unit SizeEach