Thermo Fisher Scientific MEK2 Polyclonal Antibody
인간, 마우스, 랫트에 반응하는 MEK2 폴리클로날 항체로 Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체이며, 장기 보관 시 -20°C에서 보관 권장. 연구용으로 세포 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MEK1/MEK2 (aa 18–234). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546767 |
Product Specific Information
- Recommended positive controls: MEK1-transfected 293T, MEK2-transfected 293T, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a key role in mitogen growth factor signal transduction. MEK2 (MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, forming a negative feedback loop that suppresses MEK1 activation.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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