Thermo Fisher Scientific MCAM Monoclonal Antibody (OTI2B2), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD146 (MCAM) is an integral membrane glycoprotein of the immunoglobulin superfamily. It is expressed at cell-cell junctions in endothelial cells and various other normal and malignant cell types. CD146 functions as a calcium-independent adhesion molecule and may participate in signal transduction by recruiting Fyn kinase and promoting tyrosine phosphorylation involved in actin cytoskeleton assembly.
It plays a key role in endothelial cohesion and vascular integrity. CD146 expression in melanoma cells may facilitate interactions with vascular elements, enhancing metastasis. Additionally, CD146 may function as an adhesion molecule in neural crest cells during embryonic development and acts as a surface receptor inducing phosphorylation of FYN and PTK2, leading to increased intracellular calcium levels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.