Thermo Fisher Scientific MCAM Monoclonal Antibody (OTI1F10), TrueMAB
MCAM 단백질을 타깃으로 하는 Mouse monoclonal antibody로, Western blot에 최적화되어 있습니다. Human 시료에 반응하며, Lyophilized 형태로 제공됩니다. PBS buffer에 trehalose가 포함되어 안정성이 높고, 연구용으로 적합합니다.
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Applications
- Western Blot (WB): 1:2,000 dilution tested
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F10 |
| Immunogen | Full length human recombinant protein of human MCAM produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD146 (MCAM) is an integral membrane glycoprotein of the immunoglobulin superfamily. It is expressed at cell-cell junctions in endothelial cells and other normal or malignant cell types.
CD146 functions as a calcium-independent cell adhesion molecule and may act in signal transduction via Fyn kinase, influencing actin cytoskeleton assembly.
It contributes to endothelial cohesion, tumor cell vascular interactions, and may play roles in embryonic neural crest development and intracellular signaling involving tyrosine phosphorylation of FYN and PTK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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