Thermo Fisher Scientific AKAP8 Recombinant Rabbit Monoclonal Antibody (23GB2970)
Recombinant rabbit monoclonal antibody targeting AKAP8 (AKAP95). Validated for WB, ICC/IF, and Flow Cytometry. High specificity and reproducibility with affinity-purified form. Suitable for human, mouse, and rat samples. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB2970 |
| Immunogen | A synthesized peptide derived from AKAP95 (30–55AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20 °C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase within the cell. The R subunit (RII) of PKA interacts with RII-anchoring proteins, collectively known as A-kinase anchoring proteins (AKAPs).
AKAP95, also known as AKAP8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney, and skeletal muscle. During mitosis, AKAP95 is recruited to chromosomes and plays an essential role in mitotic progression. It participates in PKA signaling through interaction with the RII regulatory subunit and forms a complex with HA95 and HDAC3, required for deacetylation of Histone H3 during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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