Thermo Fisher Scientific Phospho-DAXX (Ser517) Polyclonal Antibody
Phospho-DAXX (Ser517) 특이적 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 사용 가능하며, Protein A로 정제된 1 mg/mL 액상 형태입니다. -20°C 보관, 연구용 전용입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human DAXX around the phosphorylation site of Ser517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The DAXX gene encodes a multifunctional protein localized in both the nucleus and cytoplasm. It interacts with various proteins such as apoptosis antigen Fas, centromere protein C, and transcription factor E26 oncogene homolog 1. In the nucleus, DAXX acts as a transcription repressor binding to sumoylated transcription factors, with repression relieved by sequestration into promyelocytic leukemia nuclear bodies or nucleoli. It associates with centromeres during G2 phase and may regulate apoptosis in the cytoplasm. Its localization and function are influenced by post-translational modifications including sumoylation, phosphorylation, and polyubiquitination. Multiple transcript variants arise from alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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