Thermo Fisher Scientific NONO Monoclonal Antibody (OTI4D9), TrueMAB
NONO 단백질을 인식하는 Mouse monoclonal antibody로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. Human, Mouse, Rat, Dog 반응성. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 포함. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 184–385 of human NONO produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The NONO gene encodes an RNA-binding protein involved in multiple nuclear functions, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants and pseudogenes exist on Chromosomes 2 and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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