Thermo Fisher Scientific RNF144B Monoclonal Antibody (OTI3C2), TrueMAB
RNF144B 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, ICC/IF, Flow cytometry 등에 사용 가능. 인간 RNF144B(aa 1-256) 재조합 단백질로 면역화됨. 무보존제, 동결건조 형태로 제공되며, 연구용으로 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–256 of human RNF144B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RNF144B is an E3 ubiquitin-protein ligase that transfers ubiquitin from E2 enzymes (UBE2L3, UBE2L6) to substrates such as LCMT2, promoting their degradation. It induces apoptosis via a p53-dependent but caspase-independent mechanism. Overexpression decreases the ubiquitin-dependent stability of BAX, leading to reduced cell death, though RNF144B is not inherently anti-apoptotic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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