Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555

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Alexa Fluor 555 결합 Donkey anti-Rat IgG (H+L) 항체로 높은 특이성과 낮은 교차반응을 제공합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 형광 이미징 실험에 적합합니다. 고감도, 높은 안정성, 실온 배송.

카탈로그번호: A78945
브랜드: Thermo Fisher Scientific
카테고리: Polyclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific A78945 Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 1 mg pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555

Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555

Applications and Tested Dilution

Application	Tested Dilution
Western Blot (WB)	0.2–2 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))	1:2,000
Immunocytochemistry (ICC/IF)	1–2 µg/mL
Flow Cytometry (Flow)	1–4 µg/mL

Product Specifications

Specification	Description
Species Reactivity	Rat
Host / Isotype	Donkey / IgG
Class	Polyclonal
Type	Secondary Antibody
Immunogen	Gamma Immunoglobins Heavy and Light chains
Conjugate	Alexa Fluor™ 555
Excitation / Emission Max	553 / 568 nm
Form	Liquid
Concentration	2 mg/mL
Purification	Purified
Storage Buffer	PBS, pH 7.5
Contains	5 mM sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Ambient
RRID	AB_2910652
Target	IgG
Cross Adsorption	Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep serum
Antibody Form	Whole Antibody

Alexa Fluor 488
Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 647
Alexa Fluor Plus 405
Alexa Fluor Plus 488
Alexa Fluor Plus 555
Alexa Fluor Plus 594
Alexa Fluor Plus 647
AP
Biotin
FITC
HRP
TRITC
Request custom conjugation available

Product Specific Information

This donkey anti-rat IgG (H+L) whole secondary antibody is affinity-purified and shows minimal cross-reactivity to serum proteins from multiple species. Cross-adsorption enhances specificity, yielding higher sensitivity and reduced background staining.
The Alexa Fluor 555 dye provides bright orange fluorescence with excellent photostability and pH insensitivity, ideal for imaging and flow cytometry. Each conjugate typically contains 2–8 fluorophore molecules per IgG.
Before use, centrifuge briefly and use only the supernatant to minimize aggregates. Recommended final concentration for most applications: 1–10 µg/mL.
Product ships at room temperature.

Target Information

Anti-Rat secondary antibodies are affinity-purified and offer high specificity for rat immunoglobulins. They enhance detection versatility and sensitivity through signal amplification. Commonly produced by immunizing host animals with pooled rat immunoglobulins, these antibodies can be further purified or conjugated for specialized use.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.